Dressed in a cheerleader's uniform, the 53-year-old flashed her red briefs to the crowd at the Meazza stadium in Italy.
With a lift of her white miniskirt, Madonna made sure to draw attention to her scarlet undergarments and even clutched her behind.
The rest of Madonna's outfit consisted of a matching white top with red diagonal strips, red gloves and white knee-high boots.
Twirling a baton and marching around the stage, Madge made sure to give the audience some bang for their buck.
After Madonna flashing her nipple on stage in Istanbul last weekend.
Madonna whipped down her trousers and flashed her bottom in Rome on Tuesday night a few days after her nipple slip in Turkey.
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